Phosphorus, Reactive (Orthophosphate)
© Hach Company, 2003. All rights reserved. Printed in the U.S.A. 3/04 1ed
Page 4 of 4 PhosphorusReac_UniCell_Other_Asc_Eng_Ody.fm
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Method Performance
Precision
Standard: 30.0 mg/L PO
4
3–
See Section 3.4.3 Precision on page 44 for more information, or if the standard
concentration did not fall within the specified range.
Sensitivity
See Section 3.4.5 Sensitivity on page 44 for more information.
Summary of Method
Phosphate ions react with molybdate and antimony ions in an acidic solution to
form an antimonyl phosphomolybdate complex. Ascorbic acid reduces this
complex to phosphomolybdenum blue. Test results are measured at 880 nm.
Required Reagents
Description Unit Cat. No.
Phosphate - PO
4
3–
, HR, UniCell™ HCT 122................................................................... 23/pkg.............. HCT 122
Required Apparatus
Adapter, 16-mm Cell .......................................................................................................... each....................59457-00
Optional Apparatus
Cylinder, mixing, 25-mL graduated................................................................................. each....................20886-40
Digital Reactor Block 100................................................................................................... each....................DRB 100
Pipettor, Jencons, 100–1000 µL.......................................................................................... each....................27949-00
Rack, Cooling, Test Tube.................................................................................................... each....................18641-00
Tips, replacement for 27949-00 ......................................................................................... 400/pkg.............27950-00
Optional Standards
Hydrochloric Acid Solution, 1:1....................................................................................... 500 mL...................884-49
Phosphate Standard, 10-mL Voluette
®
Ampule, 500-mg/L as PO
4
............................ 100 mL...............14242-10
Phosphate Standard, 30-mg/L as PO
4
............................................................................. 946 mL...............14367-16
Program 95% Confidence Limits of Distribution
804 29.5–30.5 mg/L PO
4
3–
Program Portion of Curve ∆Abs ∆Concentration
804 Entire range 0.010 0.5 mg/L PO
4
3–