TPH (Total Petroleum Hydrocarbons)
TPH (Total Petroleum Hydrocarbons)
TPHSoil_None_Other_IMM_Eng_Ody.fm Page 3 of 12
1. Touch
Single Wavelength
then touch the λ button.
Type in 450 nm and touch
OK.
Note: Use the 1-cm square cell
holder for this procedure.
2. Label an Antibody
Cuvette for each
calibrator and each
sample to be tested.
To select the proper
calibrators, see Tab le 1 on
page 10 or Tabl e 2 on
page 10.
Note: As many as 10 cuvettes
may be tested at one time and
may comprise any combination
of samples and calibrators.
Note: See example below.
3. Place the cuvettes
into the rack snugly.
4. Pipet 0.5 mL of
Diluent Solution into
each Calibrator cuvette.
Note: The same pipette tip can
be used repeatedly for this
step.
Immunoassay Procedure for Soil Extracts and Water Samples
λ
OK
54 0
EXAMPLE
To test samples against the 50-ppm and 100-ppm diesel fuel calibrators, label
one Antibody Cuvette “50” and a second cuvette “100.” Then label an Antibody
Cuvette for each of up to eight samples to be tested. In this example, there is
room for eight samples; samples plus calibrators cannot exceed 10. Using
more calibrators will reduce the number of samples that can be run at the
same time.
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