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Hettich MIKRO 220 - Quick Stop Function; Software Operation; Centrifugation Parameters; Relative Centrifugal Force, RCF

Hettich MIKRO 220
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Rev.: 04 / 11.2023
AB2200en_SA
Software operation
2. Release the
[START/IMPULSE]
button to end the centrifugation run.
🡆 Ramp-down takes place with the set brake level. The brake level is
displayed.
An audible signal sounds when the rotor comes to a standstill.
‘OPEN’
‘OEFFNEN’
is displayed.
6.6 Quick stop function
Personnel:
â– 
Trained user
Press the
[STOP/OPEN]
button twice.
🡆 Ramp-down with brake level "9" (shortest ramp-down time) is dis-
played and executed.
7 Software operation
7.1 Centrifugation parameters
7.1.1 Relative centrifugal force, RCF
The relative centrifugal force RCF is dependent on the speed and the centri-
fuging radius.
The relative centrifugal force RCF is stated as a multiple of the acceleration
due to gravity (g).
The relative centrifugal force RCF is a dimensionless numerical value and is
used to compare the separation and sedimentation performance.
RCF = Relative Centrifugal Force
RPM = speed
r = centrifuging radius in mm = distance from the centre of the axis of
rotation to the bottom of the centrifuge tube.
7.1.2
Centrifugation of substances or mixtures of substances with a density higher than
1.2 kg/dm
3
The density of the substances or mixtures of substances must not exceed
1.2kg/dm³ during centrifugation at maximum speed. The speed must be
reduced for substances or substance mixtures with a higher density. The
permissible speed can be calculated using the following formula:
For example: Maximum speed 4000 RPM, density 1.6kg/dm³

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