C2 SINGLE/THREE CHANNEL FLUOROMETER
The innovative Single/Three-Channel Fluorometer makes available to the marine, freshwater,
surveying markets a high performance sensor providing the user with increased
functionality when compared to other standard fluorometers. The Single/Three-Channel
Fluorometer can be tailored to measure a wide range of fluorescent parameters (see the below
table). Moreover, it is possible to extend the measurement range through the use of dynamic
range function. Dynamic range can be adjusted simply by varying the LED intensity, while
still maintaining the factory set calibration via the internal referencing of LED output. All
versions of the Three-Channel Fluorometer come with a Chlorophyll a channel as standard;
two other channels can then be selected from phycoerythrin, phycocyanin or nephelometer
(turbidity) options. The Three-Channel Fluorometer allows the user to assess the relative
contribution to Chlorophyll a fluorescence emission from the different light harvesting pigments
absorbing light at each of the chosen excitation wavelengths. This information can then be used to make
an assessment of the different classes of phytoplankton present in the sample under analysis.
Features
➢ Range of wavelengths available.
➢ High rejection of ambient daylight.
➢ Low noise high sensitivity.
➢ Low turbidity breakthrough.
➢ Low power consumption.
➢ Rugged corrosion free “Acetal C” housing.
➢ Internal referencing of excitation intensity.
Applications
➢ In Situ Chlorophyll & Algal class study.
➢ Environmental monitoring.
➢ Particulate studies.
➢ River and Stream monitoring.
➢ Pollution monitoring.
➢ Water and Wastewater quality
Fluorescent Parameter Dynamic Range
(*)
Limit of detection
Chlorophyll-a 0..100 µg/L < 0.01 µg/L
Fluorescein 0..100 µg/L < 0.005 µg/L
Rhodamine WT 0..100 µg/L < 0.02 µg/L
Phycoerythrin 0..100 µg/L < 0.02 µg/L
Phycocyanin 0..100 µg/L < 0.01 µg/L
Nephelometer (Turbidity) 0..100 FTU < 0.02 FTU
(*) User configurable up to 500 µg/Lor 500 FTU
C2.1 CALIBRATION
The Single/Three-Channel Fluorimeter can be re-calibrated by the user using the procedure described
in the dedicated Fluorimeter Operator’s Manual. The calibration is done by using the dedicated interface
software. Therefore, before starting the calibration operations, it is mandatory to install the software in
a personal computer. This section describes the step-by-step procedure to run the software on the PC to
communicate with the Single/Three-Channel Fluorimeter installed in the OCEAN SEVEN 310 probe.
1 – Connect the OS310 to a personal computer with the RS232 laboratory cable.
2 – Run the ITERM program.
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