NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 15 / 70
4.1.2 Analysis of dsDNA, ssDNA and RNA
The procedure is as follows:
Parameter Screen
NanoVolume Applications
Cuvette Applications
Parameter Screen
Step 1 Press 1 for NanoVolume OR 2 for Cuvette folder
Step 2 Press 1 to select Nucleic Acids folder
Step 3 Press 1 to select dsDNA mode OR 2 to select ssDNA
mode OR 3 to select RNA mode
Step 4 Using the NanoVolume Applications select the Lid
Factor as described in the “Average Detection Range
Sheet” and under 3.2.
Step 5 Enter the Dilution Factor using the keypad numbers.
Range 1.00 to 9,999. Use the C button to backspace
and clear the last digit entered OR press Menu/Options
to enter the dilution factor screen. Enter the volume of
the sample using the keypad numbers. Range 0.01 to
9,999. Enter the volume of the diluent using the keypad
numbers. Range 0.01 to 9,999. Press OK to
calculate the dilution factor and return to the
Parameters screen OR press Cancel to cancel the
selections and return to the Parameters screen.
Step 6 Background correction at 320 nm is recommended to
be switched On.
Step 7 Select the Units of measurement using the left and right
arrows. Options: μg/ml, ng/μl, μg/μl.
Step 8 Enter the Factor using the keypad numbers. Default
value is 50 for dsDNA, 37 for ssDNA and 40 for RNA,
range is 0.01 to 9,999.
Step 9 Press OK to enter the Results screen OR Cancel
to return to the Nucleic Acids folder.
Results Screen
Results Screen
Step 10 Apply/insert the reference sample. Press the Blank Key.
This will be used for all subsequent samples until changed.
Step 11 Apply/insert sample and press Sample . This measures
at the selected wavelengths and displays the results. The
sample concentration, the ratio of A260/A280 and
A260/A230 are calculated (corrected by the background
wavelength value if selected).
Step 12 If the absorbance value of the sample is not in the linear
range a “Warning message” will pop up and “Instruction”
will be displayed in the top left corner of the result screen.
Please refer to 3.2 Software instructions/important
information on page 11 for further information.
Step 13 Repeat for all samples.
Step 14 Press Menu/Options to display available options which are
described on page 8.
Step 15 Press Escape and confirm with Yes to return to the
Nucleic Acids folder.
To change parameters, print or save methods press the Menu/Options button. The options menu will be opened. For
further explanation please see 2.3 Keypad and display on 6 (P 300) and 7 (P330 / P 360).
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