NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 39 / 70
5.1 Single Wavelength – Abs and %T
This makes simple absorbance (A) and % transmission (%T) measurements on samples, measuring the amount of light
that has passed through a sample relative to a reference (this can be air).
The procedure is as follows:
Parameter Screen
Results Screen
Parameter Screen
Step 1 Press 3 to select Functions.
Step 2 Press 1 to select Single Wavelength
Step 3 Set Wavelength by using keypad numbers or left and right
arrows.
Step 4 Select the Mode, Absorbance or %Transmission, using the
left and right arrows.
Step 5 To enter the results screen with the selected parameters
press OK OR cancel the selections and return to the
Functions folder by pressing Cancel .
Results Screen
Step 6 Insert the reference sample. Press Blank key. This will be
used for all subsequent samples until changed.
Step 7 Insert sample and press Sample .
Step 8 Repeat for all samples.
Step 9 The result at the selected wavelength is displayed on the
screen.
Step 10 Use the left and right arrows to move the cursor and
display the value at the cursor position (+/- 15nm from
set wavelength).
Step 11 Press Menu/Options to display available options which
are described below.
Step 12 Press Escape and confirm with Yes to return to the
Functions folder. Query needs confirmation to avoid
unintended escaping the application folder.
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