Implen P 330 User Manual

NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 6 / 70
2. INTRODUCTION
2.1 Your spectrophotometer
Your spectrophotometer is a simple-to-use UV/Visible instrument with a CCD array detector (1024 pixels). It has no
moving parts, which is the basis of the rapid scanning operating system.
The user interface is built around folders which are displayed on the main screen when the instrument is switched on.
Different folders are numbered and opened by using the associated number key on the keypad. After switching on the
NanoPhotometer
®
a self-calibration check is performed and the default main screen "NanoPhotometer
®
” is offering
the choice of:
Keypad
number
Description
1
Life Science methods such as nucleic acid assays
and protein assays using the NanoPhotometer
®
P-
Class Submicroliter Cell
2
Life Science methods such as nucleic acid assays,
protein assays and cell density using cuvettes
3
General spectroscopic methods
4
Contains nine folders that can store user adapted
methods (up to 81)
5
Instrument set up (date, time, number format),
Output Options and Baseline Compensation set up.
The instrument is equipped with a standard USB port (back) and the P330/P360 also with a USB flash drive port (right
side). The NanoPhotometer
®
P-Class Software Package is necessary to connect the NanoPhotometer
®
P-Class to a PC
or to read the saved data on the USB flash drive. The software enables the user to “print through” the PC directly to the
printer that is connected to it. Data may be stored as Excel spreadsheet (report and/or table format), EMF graphics file,
a comma delimited (csv) data file, a tab delimited (txt) data file or in native NanoPhotometer
®
P-Class Software format
for later access (see also NanoPhotometer
®
P-Class PVC Installation and User Manual).
Alternatively, results may be saved on a SD Memory Card (P300 only) or sent to the PC via a Bluetooth (P300 only)
accessory; these can either be supplied pre-installed or are available as an optional accessory if the need for the use
arises after installation of the product.
A thermal built-in printer is available for the instrument; this may either be supplied pre-installed or is available as an
optional accessory if the need for its use arises after installation of the product.
2.2 Sample handling tips
 The NanoPhotometer
®
P-Class includes an integrated vortexer (P 330 / P 360 only) to assure a good homogeneity
of the sample. It is recommended to mix every sample before a measurement.
 Note that the light beam is directed from RIGHT to LEFT through the cell chamber; therefore please ensure the
measurement cell is inserted in the correct alignment.
 Insert the measurement cell always in the same direction.
 The cell holder supplied with the instrument accepts the NanoPhotometer
®
P-Class Submicroliter Cell and standard
10 mm pathlength quartz, glass or plastic cells.
 The optical height of the NanoPhotometer
®
P-Class is 15 mm.
 The minimum volume that can be used is 0.3 µl with the NanoPhotometer
®
P-Class Submicroliter Cell.
 12 mm test tubes may be used (e.g. for cell cultures), however they are not recommended as higher quality data is
produced by using disposable cuvettes for the analysis. If used, align the indicator line on 12 mm test tubes in the
same direction to ensure reproducible positioning of the tube. Note that test tubes do not last forever, and that the
surface becomes scratched and blemished through repetitive use; if this is the case they should be replaced.

Implen P 330 Specifications

General

Brand	Implen
Model	P 330
Category	Measuring Instruments
Language	English