NanoPhotometer
®
P-Class User Manual
Version 2.1 Page 49 / 70
Standard Screen
Calibration Screen (replicates off)
Calibration Screen (replicates on)
Standards screen
Step 10 Enter the concentration values by using the keypad
numbers and the up and down arrows to move between
the different standard boxes. Range 0.001 to 9,999.
Step 11 Press Next to enter the Calibration screen. If any
duplicate or non-monotonic (increasing entries) are
present the unit will beep and highlight the incorrect
entry. OR press Back to return to the Parameter
screen.
Calibration Screen (replicates off)
Step 12 This shows the calibration values and allows standards to
be measured.
Step 13 Insert the reference sample. Press Blank key. This will be
used for all subsequent samples until changed.
Step 14 Insert the standard (use C to clear previously stored
results before measuring) and press Sample to
measure the standard and store the result.
Step 15 Repeat for all standards. A graph will display the results
and the fitted curve as the measurements are input.
Step 16 Use the up and down arrows to select a standard to be
repeated if a poor reading has been obtained. Use C to
clear the previous reading.
Step 17 Press OK to accept the calibration and go to the
Results screen (see below) OR press Back to return to
the Standards screen.
Calibration Screen (replicates on)
This shows the calibration values and allows standards to
be measured.
Step 18 Insert the reference sample. Press Blank key. This will be
used for all subsequent samples until changed.
Step 19 Press Replicates to display the replicate entry boxes.
Use C to clear previously stored results before measuring.
Step 20 Insert the standard and press Sample to measure the
standard and store the result.
Step 21 Repeat for all replicates and standards. A graph will
display the results and the fitted curve as the
measurements are input.
Step 22 Use the up and down arrows to select a standard to be
repeated if a poor reading has been obtained. Use C to
clear the previous reading.
Step 23 Press Next to accept the calibration and go to the
Results screen (see below) OR press Back to return to
the Standards screen.
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