Nikon DIAPHOT 300 - Photomicrography.39

19. Photomicrography
1) Checking before photomicrography
Before starting photomicrography, ensure the following:
(1) Eliminate illumination unevenness.
Make sure that:
• The condenser is centered. (See page 7.)
• The condenser annular diaphragm is centered (for phase contrast
microscopy). (See page 9.)
• The aperture of the condenser aperture diaphragm is proper. (Normally 70
to 80% of objective's N.A.). (See page 22.)
• The field aperture diaphragm is stopped down to a range slightly larger than
the photomicrography frame. (See page 21 .)
(2) Set the voltage and filter properly. (Seepage 17.)
Lamp voltage Filter
Daylight type color film PHOTO
Put NCB1 1 into optical path.
Tungsten type color film
8 Remove NCB1 1 .
Monochrome film
6 or more
Remove NCB1 1 .
Use color correction (CC) filters obtainable on the market if necessary.
(3) Focus the microscope properly. (See page 40.)
(4) Prevent external light from entering. (See page 14.)
2) Relationship between photomask and photography range
(1) 35mm SLR camera (on front port)
The rectangular frame of the photomask shows the range of photography.
The magnification on the film surface is the objective's magnification
multiplied by 2.5 (the intermediate magnification of the microscope). If
higher magnification is needed, use the conversion lens CL2 (option). Note
that a magnification obtained by using an objective only is better in resolution
than the same magnification obtained by combining a conversion lens with an
objective.
(2) Photomicrographic attachment
The rectangular frame of the photomask shows the photomicrography range
of a photomicrographic attachment used with the 2.5 x PL lens. If a PL lens
of different magnification is used or the photomicrography range should be
determined accurately, determine the photomicrography range by looking
through the finder of the photomicrographic attachment. (Refer to the
instruction manual of the photomicrographic attachment.)