Nikon DIAPHOT 300 - Troubleshooting

YU. TROUBLESHOOTING
If the microscope does not function properly, take appropriate action as described
below.
1. Optical
Symptoms Causes
Countermeasures
Viewfield
vignetting, uneven
brightness, or
viewfield only
partially visible
Optical path changeover dial
not in clickstop position
Rotate to clickstop position.
Revolving nosepiece not
correctly mounted on focusing
mechanism
Mount securely.
Revolving nosepiece not in
clickstop position (Objective not
in optical path)
Set to clickstop position.
Slider in focusing mechanism
not in clickstop position
Set to clickstop position.
Incorrect condenser mounting Mount correctly.
Incorrect condenser turret
positioning
Correctly set to the position that
matches the objective.
Incorrect turret assembly
positioning
Set turret to [0] position.
Photomask in intermediate
position
Turn reticle in/out lever until it
clicks at stopper.
Stage ring in optical path
Change specimen position and
move stage.
Field aperture diaphragm image
not in focus on specimen
surface
Rotate condenser focus knob to
bring field aperture diaphragm
image into focus on specimen
surface. (For LWD condenser
with supplementary lens,
loosen refocusing stopper
before this operation.)
Field aperture diaphragm
stopped down excessively
Open diaphragm properly.
Dirt or dust on lens (field lens,
condenser, eyepiece) or culture
container
Clean lens or culture container.
Dirt or dust in
viewfield
Dirt or dust on lens (field lens,
condenser, eyepiece) or culture
container
Clean lens or culture container.
Field aperture diaphragm image
not in focus on specimen
surface
Rotate condenser focus knob to
bring field aperture diaphragm
image into focus on specimen
surface. (For LWD condenser
with supplementary lens,
loosen refocusing stopper
before this operation.)
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