Nikon DIAPHOT 300 - Photomicrographic Procedure

Ill . PHOTOMICROGRAPHIC PROCEDURE
Nikon's 35mm single-lens reflex cameras (such as F-601) and photomicrographic
attachment MICROFLEX Series (option) may be used for photomicrography with the
Diaphot 300.
Mount an SLR camera directly on the microscope front port. Because intermediate
magnification, 2.5x, is applied, the photomicrography magnification is the
"magnification of the objective multiplied by 2.5". The use of a cable release
(option) eliminates the effects of vibration, allowing efficient photomicrography.
Mount a photomicrographic attachment directly on the side port, or on the eyepiece
tube port via the optional eyepiece tube bracket and the trinocular eyepiece tube for
the upright type microscope (option).
The following shows the photomicrographic procedures using a 35 mm SLR
camera.
Before starting photomicrography, make sure that:
♦ The microscope is ready for normal microscopy. (See page 4.)
♦ The camera is loaded with film. (Refer to the instruction manual that comes with
your camera.)
♦ For the photomicrographic procedures using the photomicrographic attachment,
refer to its manual. Rotate the optical path changeover dial to align the optical
path to the port on which the photomicrographic attachment is mounted.
♦ Regarding the procedure for mounting a camera or photomicrographic device, see
Section V . "ASSEMBLY", from page 42.
♦ For detailed operations with individual components, see Section IV .
"OPERATIONS ON COMPONENTS ", from page 16.
1. Turn on the camera.
Flip on the main switch of the camera.
Set the camera for the appropriate film
sensitivity, exposure mode, and
photometry mode. (Refer to the
instructions supplied with the camera.)
Figure 3-1
2. Adjust lamp voltage to PHOTO.
Set the light intensity control dial on the
left side of the base to PHOTO, (page 1 7)
(If the light intensity control dial on the
power supply unit is used, set near "9".)
Figure 3-2