Nikon DIAPHOT 300 - Page 17

18. Perform microscopy with a desired phase contrast objective.
• Use the annular diaphragm (module) that has the same Ph code as marked on
the objective on the optical path. Every time the annular diaphragm (module)
is changed, center the annular diaphragm as described in 1 7 . (As for the
ELWD condenser, once centering is complete with PhL, other annular
diaphragms are also centered automatically.)
• Adjust the field aperture diaphragm until it is circumscribed with the viewfield.
(Open it to the full for the SLWD condenser)
• Adjust the brightness with the ND filters or the light intensity control dial.
• If the system condenser turret unit is used, always open the condenser
aperture diaphragm to the full.
• For microscopy at the maximum contrast, bring the GIF filter into the optical
path. Keep the NCB1 1 filter away from the optical path when using GIF filter.
• When replacing the specimen, you can use the refocusing stopper (page 34),
condenser refocusing stopper (page 35), and pillar tilting mechanism (page
36) for enhanced efficiency.
Now, the phase contrast microscopic operation is all completed.
When you observe a specimen in a laboratory dish or other containers, we
recommend the use of an objective that has a correction ring for compensating the
thickness of the dish or container bottom. (See page 26.)