Nikon DIAPHOT 300 - Page 57

Symptoms Causes
Countermeasures
Viewfield too bright
No ND filter in optical path
Insert ND filter(s) into optical
path.
Too high voltage setting with
light intensity control dial
Set to lower voltage with light
intensity control dial.
Viewfield too dark
Condenser aperture diaphragm
stopped down excessively
Open diaphragm properly.
Field aperture diaphragm image
not in focus on specimen
surface
Rotate condenser focus knob to
bring field aperture diaphragm
image into focus on specimen
surface.
Optical path changeover dial set
to B or D
Set optical path changeover dial
to A.
2. Operational
Symptoms Causes Countermeasures
Focusing
impossible with
objective raised to
highest position
Stage mounted incorrectly Mount correctly.
Focusing
impossible with
20 x or 40 x
objective
Culture container glass (or
plastic) thicker than 2mm
Use glass (or plastic) not
thickern than of 2mm
Binocular images
not coincident
Incorrect interpupillary
adiustment
Adjust interpupillary distance.
Eye fatigue
experienced during
observation
Incorrect diopter adjustment Correct diopter adjustment.
Inadequate illumination
brightness
Correct lamp voltage. Or,
adjust with ND filter.