Nikon ECLIPSE 80i - Page 32

Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)
2.3 Bright-Field Microscopy
30
13
Switch to the desired objective
and view the specimen.
Adjust the field diaphragm and
aperture diaphragm each time
you change objectives.
F
ield dia
p
hra
g
m:
Set slightly narrower than the margins of
the field of view.
Ap
erture dia
p
hra
g
m:
70% to 80% of the maximum numerical
aperture (N.A.) of the objective
14
Turn off power after completing
observations.
Select the desired ob
j
ective.
Press the
p
ower switch
to the "◯" position.
Aperture diaphragm
knob
Field dia
p
hra
g
m knob