Chapter 2 Part Names and Microscopy Procedures (With Epi-illumination Attachment Mounted)
2.4 Epi-fluorescence Microscopy
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2
10
Focus on the specimen.
11
Center the field diaphragm of the
Epi-illumination attachment.
Refer to "4. Epi-fluorescent Field Diaphragm" in
"17. Fluorescence Observation" of the separate
Reference manual.
12
Switch to the desired objective
and view the specimen.
• Use the ND filters for the fluorescence
attachment to adjust brightness.
• Adjust the field diaphragm so that it extends
slightly beyond the field of view.
• The image brightness can be adjusted with
the aperture diaphragm. Complete centering
before using the aperture diaphragm.
• When using oil immersion objectives, apply
immersion oil between the specimen and the
objective.
Focus on the specimen using
the coarse and fine focus knobs.
Field
diaphragm
image
Field diaphragm
open/close lever
Field diaphragm
centering screw holes
Eyepiece
field of view
ND4 filter slider
ND16 filter slider
ND8 filter slider
Select the desired
objective.
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