Nikon ECLIPSE 80i - Page 48

Chapter 3 Part Names and Microscopy Procedures(With DIH-M Mounted)
3.4 Epi-fluorescence Microscopy
46
6
Switch on power for the C-Box
and Epi-illumination light source.
(Note that the shutter for the
Epi-illumination is closed
automatically.)
7
Press the hand switch to open
the shutter of the
Epi-illumination and center the
lamp. (Refer to the lamp
operating manual.)
8
Set the 10x objective into the
optical path.
9
Set a specimen and move the
portion to be viewed into the
optical path.
Select the 10x ob
j
ective.
Set a s
p
ecimen and secure in
p
lace
using the specimen holder.
Move the portion to be viewed into the
optical path using the XY stage knobs.
C-Box
power switch
Push to o
p
en the shutter.