IV Microscopy (Detailed Procedure)
20
Method 2: When there is no crosshairs on
your eyepiece.
Slightly lower the condenser from its uppermost
position.
(1) Swing the 40x objective in the optical path.
Rotate the coarse and then fine focus knobs
to bring the specimen in focus.
(2) Switch back to the 10x (or 4x) objective.
While looking into the right eyepiece with
your right eye, focus on the specimen by
rotating the right diopter ring and not using
the focus knob.
(3) While looking into the left eyepiece with your
left eye, focus on the specimen by rotating
the left diopter ring and not using the focus
knob.
(4) Repeat the steps (1) to (3).
7
Objective Selection
Rotate the revolving nosepiece to the desired
objective magnification.
(The objective will click into place when rotated into
position.)
Adjust the aperture diaphragm lever according to
the selected objective.
40x
Focus with this
knob.
And focus with
this ring.
Use your left
eye.
Then focus
with this ring.
Swing the
10x in the
optical path.
Use your
right eye.
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