7: Calibration
62 225-00000-000-11-201604
X Variable 2-1.174416E-05 8.35279E-07
X Variable 3-2.523787E-09 2.656608E-10 Second coefficient
Third coefficient
7. Record the Intercept, as well as the First, Second, and Third Coefficients. Additionally,
look at the value for R squared. It should be very close to 1. If not, you have most likely
assigned one of your wavelengths incorrectly.
Keep these values at hand.
Saving the New Calibration Coefficients: USB Mode
Ocean Optics programs wavelength calibration coefficients unique to each Flame onto an
EEPROM memory chip in the Flame.
You can overwrite old calibration coefficients on the EEPROM
if you are using the Flame via the
USB port.
► Procedure
To save wavelength calibration coefficients using the USB mode, perform the following
steps:
1. Ensure that the Flame is connected to the computer and that you have closed all other
applications.
2. Point your browser to http://www.oceanoptics.com/technical/softwaredownloads.asp
and scroll down to Microcode. Select USB EEPROM Programmer.
3. Save the setup file to your computer.
4. Run the Setup.exe file to install the software. The Welcome screen appears.
5. Click the Next button. The Destination Location screen appears.
6. Accept the default installation location, or click the Browse button to specify a
directory. Then, click the Next button. The Program Manager Group screen appears.
7. Click the Next button. The Start Installation screen appears.
8. Click the Next button to begin the installation. Once the installation finishes, the
Installation Complete screen appears.
9. Click the Finish button and reboot the computer when prompted.
10. Navigate to the USB EEPROM Programmer from the Start menu and run the
software.
11. Click on the desired Flame device displayed in the left pane of the USB Programmer
screen.
12. Double-click on each of the calibration coefficients displayed in the right pane of the USB
Programmer screen and enter the new values acquired in Steps 5 and 6 of the
Calibrating the Wavelength of the Spectrometer section in this appendix.
13. Repeat Step 12 for all of the new values.
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