Chapter 4
Sample Experiments
The following sections contain information on conducting sample experiments using the NIR
Spectrometer and OOIBase32.
Preparing for Experiments
► Procedure
Follow the steps below to configure the NIR Spectrometer and OOIBase32 for experiments:
1. Double-check that you have correctly installed the NIR Spectrometer, installed OOIBase32, and
configured the light source and other sampling optics.
2. Open the OOIBase32 application.
3. Select Spectrometer | Configure from the menu bar and double-check that A/D Interface
settings are correct.
4. Check your spectrometer setup configurations in OOIBase32:
a. Locate the Wavelength Calibration Data sheet that came with the HR4000.
b. Select Spectrometer | Configure from the menu and choose the Wavelength Calibration
page.
c. For each spectrometer channel in the system, enable the channel and make sure the First
Coefficient, Second Coefficient, Third Coefficient and Intercept correspond to those of the
system.
5. Adjust the acquisition parameters using the Acquisition Parameters dialog bar or select
Spectrum | Configure Data Acquisition from the menu.
If you have followed the previous steps and started OOIBase32, the spectrometer is already
acquiring data. Even with no light in the spectrometer, there should be a fluctuating trace near the
bottom of the graph. If you put light into the spectrometer, the graph trace should rise with
increasing light intensity. If this occurs, the software and hardware are correctly installed.
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