4: Sample Experiments
Transmission Experiments
Transmission is the percentage of energy passing through a sample relative to the amount that passes
through the reference. Transmission Mode is also used to show the portion of light reflected from a
sample. Transmission and reflection measurements require the same mathematical calculations. The
transmission is expressed as a percentage (%T
λ
) relative to a standard substance (such as air). The
software calculates %T (or %R
λ
) by the following equation:
S
λ
- D
λ
%T
λ
=
R
λ
- D
λ
x 100%
Where:
S
λ
= the sample intensity at wavelength λ
D
λ
= the dark intensity at wavelength λ
R
λ
= the reference intensity at wavelength λ
The following figure shows a typical transmission setup. The light source sends light via the input leg of a
transmission probe into a container. The light interacts with the sample. The output leg of the
transmission probe carries the information to the spectrometer, which transmits the information to the PC.
Common applications include measurement of transmission of light through solutions, optical filters,
optical coatings, and other optical elements such as lenses and fibers.
►
Procedure
Perform the following steps to take a transmission measurement using OOIBase32:
1. Verify that OOIBase32 is in scope mode by clicking the scope mode icon on the toolbar, or by
selecting Spectrum | Scope Mode from the menu bar.
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