Orange Photonics LIGHTLAB - Description; Causes and Fixes

TROUBLESHOOTING
Descripon
LightLab has integrated air bubbles detection that takes place during system warmup as
well as during zeroing, solvent replace and column replace. You may see a warning that
air bubbles or drift were detected and the instrument will attempt to remove the
bubbles automatically. If the air bubbles cannot be removed after three attempts during
zero, the “Zeroing Failed, Air bubbles detected” error will display. This error can occur
either during zeroing or sampling. The error indicates that an air bubble was detected
that was severe enough to have the potential of causing erroneous results.
Causes and Fixes
1. The solvent may have run out. Check the solvent level, and if it is empty replace
the solvent and press “Try Again”.
2. If the analyzer has been pumped dry for shipping, or a large bubble was
introduced by disturbing the analyzer during analysis, simply press “Try Again”.
Typically, a second attempt will correct the problem. In rare cases a third
attempt may be necessary.
3. The connections may be incorrect, loose or damaged. Check to make sure all
fluid connections are tight including the connections at the sample and waste
bottles and the column connections. If a fluid tubing is damaged, extra fluid
tubing is included. To replace, pull out old tubing from the connectors, cut the
new tubing to size and press firmly into the connector barbs.
4. Solvent was replaced without running “replace solvent” program located in
settings. Simply press “Try Again” if this was the case. The new zero will provide
sufficient sample flushing to reduce the chances of another bubble occurring.
5. Column was replaced without running “replace column” program. New columns
are shipped dry, so they need to be flushed with solvent before the first use. On
rare occasions, even after sample flushing a bubble may occur. Simply press “Try
Again” if this was the case. The new zero will provide sufficient sample flushing
to reduce the chances of another bubble occurring.
6. Incorrect sample preparation may also cause this error since very high
absorbance may trigger a bubble detection. Make sure the correct amount of
sample and solvent were used. If the incorrect sample preparation was
completed, the sample will have to be re-extracted.
7. The instrument has a clog. Flush the sample input (Settings  Flush System 
Clear Sample Input) and then re-start the analysis. If bubbles are still detected,
flush the instrument forward (Settings  Flush System  Flush Forward). If
bubbles are still detected, flush instrument backward (Settings  Flush System
 Flush Backward). Contact Support if clog persists.