7. Operating the IVIS
®
Spectrum
38
CAUTION! Caliper recommends using the XGI-8 Gas Anesthesia System
(Figure 7.3) when imaging small animals. This system supplies a controlled
amount of isoflurane to the imaging chamber and continuously reduces the
build-up of isoflurane in the chamber. If you want to use a gas other than the
recommended isoflurane/oxygen gas mixture or pure air, contact IVIS
Imaging Systems Technology Support.
Be careful to use only tubing and other plumbing fixtures that do not fluoresce
or phosphoresce (glow) in the imaging chamber. Contact IVIS Imaging
Systems Technology Support for a list of acceptable materials.
The IVIS Spectrum is equipped with plumbing connections for supplying
isoflurane anesthetic gas to the imaging chamber and exhausting gases out. The
flow of gas into the imaging chamber is manually controlled by the gas valve
on the front panel of the console. The imaging chamber does not include a gas
scavenging system. This is provided by suitable auxiliary equipment such as the
XGI-8 Gas Anesthesia System.
Gas ports are located on the rear of the system console and are labeled GAS IN
and GAS OUT. GAS IN means the direction of flow is into that port.
Similarly, the port labeled GAS OUT means that flow can be exhausted out
of this port. The flow of gas from the rear panel GAS IN port is directed to the
main gas control valve on the front of the system and proceeds to a distribution
block located on the imaging platform where it exits through the port labeled
GAS.
Figure 7.3 XGI-8 Gas Anesthesia System
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