Functional description
26
Figure 30. Laser excitation from above to adjacent well/reading from
above.
Both excitation and emission occur from the top of the sample. Bottom
reading is not possible with AlphaScreen in EnVision.
Only one measurement per well is recommended because the sample is
partially bleached by the excitation light.
Crosstalk is a factor that must also be taken into account. There are three
components: bleaching caused by the excitation of adjacent samples,
afterglow from a previously excited adjacent sample and glow from an
adjacent well at the time of measurement. A crosstalk correction wizard
allows the amount of the crosstalk to be measured and the software uses this
as a correction factor in the calculation of the final result.
TRF laser mode
Figure 31. Laser excitation (second light source) from above/reading from
above. Bias mirror module with a single mirror is used.
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