PerkinElmer EnVision User Manual

Contents
i
Contents
Introduction...............................................................................................................................3
Conventions used.................................................................................................................... 4
Functional description..............................................................................................................7
Application information.......................................................................................................... 7
Reporter gene assays ........................................................................................................... 7
Enzyme assays..................................................................................................................... 7
Time-resolved fluorometry.................................................................................................. 7
Receptor ligand binding assays........................................................................................... 7
Cellular assays..................................................................................................................... 8
Genotyping assays............................................................................................................... 8
Image FlashPlate™ assays.................................................................................................. 8
AlphaScreen assays............................................................................................................. 8
Assays with dispensing ....................................................................................................... 8
Technologies........................................................................................................................... 9
Time-resolved fluorescence (TRF) (240-850 nm) .............................................................. 9
Fluorescence intensity (240- 850 nm)................................................................................. 9
Fluorescence polarization (450-750 nm)............................................................................. 9
Luminescence...................................................................................................................... 9
Ultra Sensitive luminescence .............................................................................................. 9
Absorbance (240-950 nm)................................................................................................... 9
AlphaScreen/HTS AlphaScreen (520-620 nm)................................................................... 9
Mechanical design overview ................................................................................................ 10
Temperature control .......................................................................................................... 11
Plate types.......................................................................................................................... 11
Plate height sensor............................................................................................................. 11
Alternative plate loading modes........................................................................................ 11
Flexible stacker.................................................................................................................. 11
Robotic loading ................................................................................................................. 11
Optical system ...................................................................................................................... 12
Luminescence mode.......................................................................................................... 13
Ultra Sensitive luminescence mode .................................................................................. 14
Fluorescence Intensity....................................................................................................... 14
TR-fluorescence mode ...................................................................................................... 17
Fluorescence polarization mode........................................................................................ 21
Absorbance mode.............................................................................................................. 23
AlphaScreen / HTS AlphaScreen mode............................................................................ 25
TRF laser mode ................................................................................................................. 26
Light sources ..................................................................................................................... 27
Optical filters..................................................................................................................... 27
Focus point adjustment...................................................................................................... 28
Application specific mirror modules................................................................................. 28
Detectors............................................................................................................................ 28
Apertures ........................................................................................................................... 29
Test plate ........................................................................................................................... 29
Measurement features........................................................................................................... 29
Fast dual TRF.................................................................................................................... 29
Multiple window TRF....................................................................................................... 29
Dual ratio measurements................................................................................................... 30
Fast FP............................................................................................................................... 30

PerkinElmer EnVision Specifications

General

Light Source	Xenon flash lamp
Software	EnVision Manager
Read Modes	Absorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence, Fluorescence Polarization
Plate Formats	384-well
Detection Technology	PMT
Temperature Control	Ambient to 45°C