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PerkinElmer EnVision User Manual

PerkinElmer EnVision
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Functional description
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importance in energy transfer applications especially when the actual energy
transfer might be affected.
Dual ratio measurements
Certain labels require dual excitation or emission readings. In Wallac
EnVision protocols you can define many different labels. The instrument
will carry out the measurement quickly without any worries about any
internal calibrations of serial PMTs.
Simultaneous dual emission measurement with two emission detectors
allows you to obtain the fastest possible results.
Fast FP
Extremely fast measurement is based on a two-detector system, which
means that both polarization planes can be measured at the same time.
AlphaScreen
AlphaScreen enables very high sensitivity measurements with very low
background and very high signal to background ratio. A laser and
AlphaScreen coated-bead technology are used.
Large wavelength regions
Large wavelength ranges are needed in DNA and protein qualification
assays. EnVision supports wavelengths from 240 nm to 850 nm.
Luminescence mode
When a luminescence sample is measured, no excitation components are
used.
Minimized crosstalk in luminescence measurements
The Ultra Sensitive Luminescence feature permits the detector to be
lowered so that it is just above the plate thus reducing the crosstalk between
wells.
Crosstalk correction for Ultra Sensitive luminescence
Ultra Sensitive luminescence allows the instrument to be optimized for
glow-type crosstalk. A special crosstalk measurement is setup and run using
a crosstalk correction wizard. The software then applies the crosstalk
correction to actual sample measurements. This correction further improves
the signal to background ratio for these measurements.

Table of Contents

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PerkinElmer EnVision Specifications

General IconGeneral
Light SourceXenon flash lamp
SoftwareEnVision Manager
Read ModesAbsorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence, Fluorescence Polarization
Plate Formats384-well
Detection TechnologyPMT
Temperature ControlAmbient to 45°C