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PerkinElmer EnVision User Manual

PerkinElmer EnVision
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Specifications
71
Light sources
The flash light source used for measurements is a UV xenon flash tube,
spectral range 230 - 1100 nm. In AlphaScreen a 680 nm diode laser is used.
For homogeneous TRF-assays a 337 nm nitrogen laser is available.
Detection units
Absorbance: Photo diode, range 230-950 nm
Luminescence, Fluorescence and TR-Fluorescence: Photomultiplier tube,
range 230 - 850 nm
Ultra Sensitive Luminescence and HTS AlphaScreen: very high sensitivity,
PMT range 300 nm - 650 nm.
Mirrors
Multiple mirror module changer provided with 4 mirror positions.
Barcoded mirrors.
Filters
Changeable excitation filter slide, provided with 8 filter positions
(Ø15.0 mm)
Changeable emission filter slide, provided with 8 filter positions
(Ø15.0 mm)
Barcoded filters.
Apertures
Three apertures are available: for 1536, 384 and 96-well plates respectively.
These are for Ultra Sensitive luminescence or HTS AlphaScreen
measurements.
A shutter is available to protect the Ultra Sensitive luminescence detector
when such measurements are not being made.
Dispenser
- Dispensing up to 384-well plates
- Volumes 2µl - 475µl
- Adjustable dispensing speed 100µl/s – 500µl/s
- Precision for 10µl volume <10%
- Dead volume below 500µl
- Simultaneous dispensing with two pumps for maximum throughput
- In real-time kinetics the dispensing can be set to occur on any selected
repeat

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PerkinElmer EnVision Specifications

General IconGeneral
Light SourceXenon flash lamp
SoftwareEnVision Manager
Read ModesAbsorbance, Fluorescence, Luminescence, Time-Resolved Fluorescence, Fluorescence Polarization
Plate Formats384-well
Detection TechnologyPMT
Temperature ControlAmbient to 45°C