Functional description
27
Figure 32. Laser excitation (second light source) from above/dual reading
from above. Bias mirror module with two mirrors is used.
The TRF laser (second light source) is a compact nitrogen laser that does
not require any additional nitrogen source. The laser produces a very narrow
(3 nm) excitation pulse at a wavelength of 337 nm. This enables short 20µs
delay times to used, improving the signal to background ratio. The
excitation pulse is also very strong making on-the-fly TRF assays feasible.
Light sources
EnVision employs a high stability, short-arc flash lamp as a common light
source (first light source) in measurements. The high-efficiency light source
has a high repetition rate for high throughput applications and it allows you
to perform faster multi-flash measurements such as time-resolved
fluorescence and fluorescence polarization. For every measurement type
you can select the number of flashes used. To ensure both the long-term and
short-term stability of measurements, the excitation energy is monitored
after every flash via a beamsplitter and reference photodiode.
In the case of AlphaScreen a laser emitting light at wavelength of 680 nm is
used.
In the case of enhanced TRF measurements, a nitrogen laser option (second
light source) emitting light at a wavelength of 337 nm may be used. This is
fixed to the back of EnVision and the light is directed to the well by means
of a light fiber and the bias mirror module.
There is no danger to the user from these lasers unless the instrument is
broken into.
Warning! Only personnel specially trained and authorized by
PerkinElmer may open the covers that require a tool.
Optical filters
A wide range of filters covers the wavelengths used in fluorometric and
photometric applications. Additional filters are available upon request.
To Next Page
Loading...
Need help?
General