Functional description
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All interference filters are pre-installed into the barcoded filter block which
is easily installed onto a ladder-type filter slide. The structure of the block
ensures that any polarizing filters used are aligned in the right direction.
Focus point adjustment
The focus point is adjustable. The measuring height is a parameter that can
be defined in your protocol. The focus point can be located at the very
bottom or at the very top of the well or anywhere between. This is very
beneficial when measuring filters plates, cell layers, membranes etc. It also
enables you to reduce sample volumes efficiently.
Application specific mirror modules
User interchangeable application specific mirror modules form a crucial part
of the optical system. The mirror modules include all application critical
components:
• a main excitation dichroic beamsplitter
• 50/50 broadband beamsplitter
• a reference beamsplitter for measurement stabilization
• an emission dichroic beamsplitter
• broadband beamsplitter for dual measurement
• a stray light aperture located between relay optics
• a special dichroic beamsplitter for AlphaScreen
• a bias mirror module for use with the second light source
All application specific mirror modules are barcoded.
For reading from above, the multiple mirror module can hold up to four
mirror modules. Any of the mirror modules can also be in the head for
reading from below but the barcode is not then read automatically.
The mirror modules used for dual channel measurements differ from those
used for single channel measurements. Dual mirror modules include a third
reflecting component - an emission beamsplitter and a stray light aperture.
Detectors
The photoluminescence detectors are red-sensitive temperature-stabilized
side-window photomultiplier tubes. For chemiluminescence and time-
resolved fluorescence measurements the detectors are used in single photon
counting mode with factory-set high-voltage and discriminator setting. For
fluorescence intensity and fluorescence polarization measurements the
detectors are used in gated analog mode with user adjustable gain (high
voltage) setting values.
Optionally a second detector can be installed behind the first one. Two
detectors increase speed of measurement which is highly advantageous in
all dual label measurements in both fluorescence polarization assays and
time-resolved fluorescence DELFIA and LANCE assays.
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