Functional description
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Simultaneous dual dispensing can be done when immediate measurement is
not required.
In cases where maximizing the signal intensity is important, dispensing can
be done with a tip which is not between the well and the measuring head.
The unit contains two pumps, one of which can be used in real-time kinetics
measurements.
Each pump comprises a motor, syringe, valve and tubing. The aspiration
tube is used to aspirate liquid from the liquid reservoir or to retrieve liquid
back to the reservoir. The output tube is connected to a tip which is part of a
tip mount. The actual position of a tip is determined by the tip mount. In the
case of a dual pump unit the tip mount has two tips, each of which is
connected to one of the pumps.
Each tip mount is identified by a barcode which is read automatically by the
system.
A tip mount has three tip positions of which two can be used at a time.
These positions are named (from left to right) “Post”, “Real time and “Pre”.
The “Real time” position is the measurement position. “Post” is the position
the well moves to after the measurement position. “Pre” is the position it is
in before moving to the measurement position. Pump connections are as
shown in the table.
Tip configuration Pump1 Pump2
Real time Real time Not used
Pre Pre Not used
Real time and Pre Real time Pre
Pre and Post Pre Post
The offset of the “Pre” and “Post” positions depends on the plate type, 384-
well or 96-well. There is a separate tip mounts for each of these plate types.
Dispensing can be done in two ways:
- first filling the syringe and then dispensing aliquots the size of the required
volume
- aspirating the required volume and dispensing it.
The second option gives better results when small volumes (< 10 µl) are
dispensed.
There are two modes of operation:
Dispense measurement – dispensing from one or both tips is followed by
measurement.
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