PerkinElmer Lambda 365 User Manual

Introduction . 9
Figure 2. Energy Absorption
Once a sample absorbs light energy, the intensity of incoming light P
0
(the energy from the
light source) reduces to P by losing some of its energy;
therefore, we define the transmittance of a sample as follows:
Figure 3. Sample Absorption
As a result of absorption by a sample, transmittance is defined as;
T = P / P
0
And Absorbance is defined as:
A = -log T = log( P
0
/ P ) Absorbance is related to c (concentration) and b (pathlength of
light) according to the Lambert-Beer law and the equation;
A=εbc
Where, ε , the molar absorption coefficient of a particular molecule, varies with the
wavelength.
Although Beer's law has several limitations, it is widely applied to quantitative analysis.

PerkinElmer Lambda 365 Specifications

General

Wavelength Range	190 - 1100 nm
Power Requirements	100-240 V AC, 50/60 Hz
Wavelength Accuracy	±0.3 nm
Wavelength Repeatability	±0.1 nm
Wavelength Reproducibility	±0.1 nm
Photometric Repeatability	±0.001 Abs
Baseline Flatness	±0.0005 Abs
Baseline Stability	<0.0003 Abs/hour (after warm-up)
Data Interval	0.1 - 5 nm
Light Source	Tungsten halogen lamp and deuterium lamp
Sample Compartment	Standard 10 mm cell holder
Detector	Silicon Photodiode