PerkinElmer Lambda 365 User Manual

10 . Lambda 365 Users Guide
Figure 4. Transmittance and Absorbance
Double Beam UV-Visible Spectrophotometer
Lambda 365 double-beam UV-Visible spectrophotometer, the light is split into two beams
before it reaches the sample. One beam is used as the reference; the other beam passes
through the sample. The reference beam intensity is taken as 100% Transmission (or 0
Absorbance), and the measurement displayed is the ratio of the two beam intensities.
Lambda 365 double-beam UV-Visible spectrophotometer has two detectors (photodiodes),
and the sample and reference beam are measured at the same time
.

PerkinElmer Lambda 365 Specifications

General

Wavelength Range	190 - 1100 nm
Power Requirements	100-240 V AC, 50/60 Hz
Wavelength Accuracy	±0.3 nm
Wavelength Repeatability	±0.1 nm
Wavelength Reproducibility	±0.1 nm
Photometric Repeatability	±0.001 Abs
Baseline Flatness	±0.0005 Abs
Baseline Stability	<0.0003 Abs/hour (after warm-up)
Data Interval	0.1 - 5 nm
Light Source	Tungsten halogen lamp and deuterium lamp
Sample Compartment	Standard 10 mm cell holder
Detector	Silicon Photodiode