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5 GETTING STARTED
For more detailed information on particular steps of FluorPen operation please refer to chapter 6.
The device can is powered with built in Li-Ion battery. Ensure the battery is fully charged by plugging it into a PC via USB cable or the
AC outlet via the USB cable and a USB adaptor (not included). Monitoring Pen can be powered from an optional battery pack (see more
in chapter 13.1).
The FluorPen is controlled using two buttons:
• Use the MENU key to scroll through sequential menu options on the digital display and to turn the device off (hold for 3 s).
• Use the SET key to turn the device on (hold for 1 sec) and select a menu option based on cursor (>) position.
5.1 PULSES DESCRIPTION AND SETTING
Flash pulse
This function serves for setting of measuring pulses intensity. The measuring pulses are weak light pulses, which are able to induce the
minimal chlorophyll fluorescence (F
0
or F
t
). It takes only 30 µs and the maximum intensity is
3,000 µmol.m
-2
.s
-1
. It means 30 µs * 3,000 µmol.m
-2
.s
-1
= 0.09 µmol.m
-2
per pulse is the maximal intensity of the flash pulse.
Super pulse
This function serves for setting intensity of the saturating pulse. Saturating light pulse is able to induce maximum chlorophyll fluorescence
(F
m
). 100% of intensity represents approximately 3,000 µmol.m
-2
.s
-1
.
Actinic pulse
This function serves for setting intensity of actinic light. It is the ambient light in which the plants are growing. 100% of intensity equals
approximately 1,000 µmol.m
-2
.s
-1
.
Pulses used in predefined protocols:
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