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REPLIGEN Spectrum KMPi User Manual

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36 400-12355-000 Rev. 03
KR2i / KMPi TFF Systems
Setup and Operation
g. PSI DecimalsFor PSI unit of measure onlyselect preferred number of PSI decimals (range
of 0, 1, and 2)
h. Bar DecimalsFor BAR unit of measure onlyselect preferred number of BAR decimals
(range of 1, 2, and 3)
i. Key TimeoutWhen “On,” if the TFF system’s soft buttons are untouched for ten minutes
while on any screen other than the Main Screen, the TFF system will automatically switch
back to the Main Screen
j. Running ChangeWhen “On,” the tubing size and pump direction can be changed while the
pump is running
k. No. of HeadsInput number of attached pump heads
l. Perm Stop First - Permeate Pump motor stops before main drive stops
m. Check Weight - Pump Mode will not start if Feed Scale Weight is less than Initial
Concentration Weight, or if the Permeate Scale Weight is more than the Initial Diafiltration
Weight
n. Scale Req 4 Man - Manual Mode will not start unless both Feed and Permeate Scales are
present
o. Slow for Valve - If Automatic Backpressure Valve is unable to control pressure, then main
drive will slow down gradually to control pressure increases especially when concentrating
viscous solutions
p. Ramp Seconds - Determines rate at which TFF system will increase main pump flow rate
linearly over desired amount of time
q. Minus ModesEnables TFF system to use weight set-points instead of CF/DV setpoints.
Once turned on KF Comm needs to be closed and reopened to have access to these modes
in the Pump Control Window.
r. Valve 2 - (on KMPi only) Select either 'Valve 2 Port' if using a secondary automatic
backpressure valve or 'KONDUiT' if using the conductivity, temperature and UV sensors
NOTE: when connecting a KONDUiT to the Valve 2 serial connection use the null serial connector
included with the KONDUiT.
s. Shear ControlWhen set to Off, after initial ramp rate the pump drive will run continuously
at set flow rate. When set to On, TFF system will increase main pump flow rate depending
on feedback from Permeate Scale to make up for permeated volume (e.g. Permeate Scale
read weights as increasing at ~200g/min, main pump flow rate will increase by 200ml/min)
t. Cond 1 K and Cond 2 K - enter the K factor printed on the conductivity sensor when using
the KONDUiT

Table of Contents

Questions and Answers:

REPLIGEN Spectrum KMPi Specifications

General IconGeneral
ModelSpectrum KMPi
ManufacturerRepligen
ApplicationsTangential flow filtration
Operating PressureUp to 100 psi
Pressure RatingUp to 6 bar (87 psi)
Membrane AreaVariable, depending on module selection
MaterialStainless steel
Data LoggingIntegrated data logging capabilities
ConnectivityEthernet
DimensionsVaries depending on configuration
WeightVaries depending on configuration
CompatibilityCompatible with various TFF membranes

Summary

Safety Precautions

Explanation of Symbols

Explains warning symbols and their meanings, including Danger, Warning, and Caution.

Specifications

KR2i Specifications

Details technical specifications for the KR2i system, including output, input, construction, and environment.

KMPi Specifications

Details technical specifications for the KMPi system, covering output, input, construction, and environment.

System Configuration and Major Components

Setup and Operation

KF Comm Pump Control Interface

Details on using the KF Comm software for system control.

Hardware Setup

Configuring system hardware parameters and settings.

Automated Mode Control Strategy

Defining set-points and control mechanisms for automated processes.

Diafiltration Set Points

Options for controlling diafiltration endpoints.

Concentration Set Points

Options for controlling concentration endpoints.

Pressure Calibration Procedure

Steps for calibrating pressure sensors for accurate readings.

Alarms and Stops Configuration

Setting up safety alarms and operational stops for the system.

System Functionality

Detailed Pump Mode Operation

In-depth explanation of various pump operating modes.

Concentration Mode

Detailed operation of the Concentration Mode.

Concentration/Diafiltration Mode

Operation of the Concentration/Diafiltration Mode.

Constant Feed Concentration Mode

Operation of the Constant Feed Concentration (CFC) mode.

Ctrl Mode Operation

Operation of the Control Mode.

Pump Head Setup and Operation

KR2i Pump Head Operation

Procedures for installing and removing KR2i pump heads.

KMPi Pump Head Operation

Procedures for installing and operating KMPi pump heads.

Auxiliary Component Setup and Operation

Auxiliary Pumps Setup

Connecting and configuring auxiliary pumps.

Automatic Backpressure Valve

Setup and operation of automatic backpressure valves.

KONDUIT System Integration

Integrating the KONDUIT unit with the TFF system.

KF Comm Software

Installing and Updating KF Comm Software and Firmware

Guide to installing and updating system software and firmware.

Troubleshooting

Common Symptoms and Remedies

Identifies common issues and provides solutions.

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