12.3 Diafiltration
Diafiltration is the washing of cells, cell debris, virus, precipates, proteins, and other materials. For
instance, this is often done as an efficient method of buffer exchange, for instance. Diafiltration is
measured by how many washes the process volume has undergone. If the process volume is 10L and
needs to be washed 5 times, then 50L of diafiltration buffer must wash through the process volume.
The TFF system's Diafiltration function relies upon feedback from both the Feed and Permeate
Scales. The TFF system's will add buffer to maintain the concentrated weight on the Feed scale. Once
a set amount of weight is reached on the Permeate Scale—which would indicate that a set amount
of Diafiltration Volumes have washed through the process volume—the TFF system will know that
the process volume has been washed a desired number of times.
Troubleshooting
1. Pressure Sensor readings
are wrong (either -9.9 psi
or >35psi when no
Pressure sensor octopus cable
not functioning or pressure
sensor broken
2. Scale not reading properly
Refer to manufacturer’s scale
manual
Refer to manufacturer’s
scale manual
Refer to pump manufacturer’s
manual troubleshooting section
4. Process ending early or
late in the DV or CF auto
modes
Input for the holdup volumes is
holdup and permeate
holdup volumes and select
if they are empty or full
when starting the process
5. Process ending early when
using the Conductivity or
UV for auto modes
Sensor not full of proper liquid
Conductivity sensor should
be filled with same buffer
that sample is in when
starting the process. UV
sensor should be zeroed
with the same buffer
For further Technical Assistance please contact Repligen/Spectrum at techserviceca@repligen.com
or 310-885-4600
To Next Page
Loading...
Need help?
General
