10% 50% 100%
Pipette without adjustment Target Volume
Theoretical comparison of target and actual values
without adjustment
In this 1-point adjustment, 50% of maximum volume
is being used to match actual and target results
In 2-point adjustment, 10% and 100% of maximum
volume are used to match actual and target results
In 3-point adjustment, 10%, 50% and 100% of
maximum volume are used to match actual and
target results
To activate the program from a memory place:
1. Press the Hotkey (H) to see the list of memory.
2. Choose a memory place by turning the adjustment wheel (A).
3. Press the operating button (B) or the right Softkey for OK to activate the
saved program.
4. The pipette is now ready to run the program. The memory place is
displayed next to the main mode name.
3.4. Setup
The pipette settings can be changed through the Setup menu. The following
sections explain the various settings and how to modify them.
In Picus® NxT models, if password protection is enabled, some settings can
only be modified using the administrator profile (see section 3.4.10).
3.4.1. Adjustment
The default length of the piston stroke in Picus® and Picus® NxT pipettes is
defined by the manufacturer. This factory setting should be used in normal
circumstances: with aqueous liquids, at standard atmospheric pressure, and
with the pipette, tip, and liquid at standard room temperature.
When these circumstances change, the accuracy of the dispensed volume may
be affected. To maintain accuracy, the Adjustment function can be used to
change the default settings to take into account environmental factors or the
type of liquid being used.
Adjustment is needed to correct the dispensed volumes in the following cases:
−when pipetting liquids with characteristics that greatly differ from water
(for example viscous or volatile liquids)
−when the temperature between the pipette, pipette tip, and liquid differs
greatly
−when the ambient air pressure differs from that of standard atmospheric
pressure
NOTE! When factory settings are replaced with adjusted settings, the pipette’s
stated performance specifications (random and systematic error) are no
longer applicable. However, when the factory settings are reactivated, the
performance specifications will once again apply.
The Adjustment function enables the user to adjust the pipette at one, two or
three calibration points. The more measurement points selected, the greater
accuracy is reached over the whole volume range of the pipette. Single-
point adjustment is recommended when pipetting a constant volume within
a range, and two or three point adjustment if performance over the whole
range is required.
The adjustment options are:
−1-point adjustment: adjustment at one point only, the user can choose the
desired adjustment point/volume
−2-point adjustment: adjustment at 10% and 100% of the nominal volume
−3-point adjustment: adjustment at 10%, 50%, and 100% of the nominal
volume
When adjusting for accuracy, the actual volumes delivered should be
measured in Pipetting mode. Once the adjustment has been made it is applied
to, all modes and an ADJ symbol is displayed on the screen.
It is possible to store three (3) adjustments in the adjustment memory: ADJ1,
ADJ2, ADJ3. These can be activated at any time when specific uses require
accuracy adjustments of the pipette.
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