Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer Getting Started Guide

Item
Guidelines
Sequence analysis sample
preparation
• Prepare sequencing reactions according to kit instructions, and purify the
extension products with ethanol precipitation, spin columns, or the BigDye
XTerminator
™
Purification Kit.
– If ethanol precipitation or spin columns are used, dry the samples in a
vacuum centrifuge without heat or at low heat for 10–15 minutes or until dry.
Note: Do not over dry the samples.
– Resuspend in 10–20 µL of Hi‑Di
™
Formamide.
• Use a 65 µL or 130 µL sample volume for samples that are prepared with the
BigDye XTerminator
™
Purification Kit. See BigDye XTerminator
™
Purification Kit
Protocol (Pub. No. 4374408).
IMPORTANT! Use the appropriate run modules for samples prepared with
BigDye XTerminator
™
Purification Kit.
• Use a 10–20 µL sample volume for samples that are prepared with Hi‑Di
™
Formamide.
• Ensure that Hi‑Di
™
Formamide is fresh.
– Hi‑Di
™
Formamide should not undergo more than two freeze-thaw cycles (one
to aliquot and one for use).
– Use the same day after thawing.
• Do not resuspend samples in water, which can decrease sample stability.
• For more information, see DNA Sequencing by Capillary Electrophoresis
Chemistry Guide Second Edition (Pub. No. 4305080).
Chapter 2 Prepare the samples and the instrument
Prepare the samples
2
10
SeqStudio
™
Genetic Analyzer Instrument and Software Getting Started Guide

Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer Specifications

General

Brand	Thermo Scientific
Model	Applied Biosystems SeqStudio Genetic Analyzer
Category	Measuring Instruments
Language	English