Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer Getting Started Guide

Prepare the samples and the
instrument
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Prepare the samples ................................................... 9
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Prepare the instrument ................................................ 11
■
Prepare and load the plate or tubes ..................................... 14
Prepare the samples
Sample preparation guidelines
Item
Guidelines
Plates and tubes
• Use MicroAmp
™
Optical 96-Well Reaction Plate or MicroAmp
™
Reaction Tubes with
a tray and retainer set.
IMPORTANT! Fast plates are not compatible with the SeqStudio
™
Genetic
Analyzer. Fast plates will damage the cartridge.
• Use the appropriate septa for plates and tubes.
• See SeqStudio
™
Genetic Analyzer Instrument and Software User Guide for more
information.
Fragment and HID analysis
sample preparation
Intensity (RFU)
Size (bp)
• Prepare the samples as recommended by the kit for fragment analysis.
• Use a 10–20 µL sample volume.
• Ensure that Hi‑Di
™
Formamide is fresh.
– Hi‑Di
™
Formamide should not undergo more than two freeze-thaw cycles (one
to aliquot and one for use).
– Use the same day after thawing.
• For more information, see DNA Fragment Analysis by Capillary Electrophoresis
User Guide (Pub. No. 4474504).
2
SeqStudio
™
Genetic Analyzer Instrument and Software Getting Started Guide
9

Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer Specifications

General

Brand	Thermo Scientific
Model	Applied Biosystems SeqStudio Genetic Analyzer
Category	Measuring Instruments
Language	English