TappingMode AFM in Fluids
11-188 Dimension 3100 Manual Rev. C
11.2 Principles of Tapping Mode in Fluids
Operation of Tapping Mode in fluid provides many of the same advantages of
Tapping Mode in air, with the additional ability to image samples under native
liquid conditions. In fluid Tapping Mode, the tip oscillates so that it only
intermittently contacts the sample surface. This reduces or eliminates frictional
forces that often damage soft samples in Contact AFM. The following sections
provide general instructions for Tapping Mode imaging in fluid, as well as
provide sample instructions for imaging RNA polymerase—a biological
sample.
Before attempting Tapping Mode in fluids, you should become familiar with
standard Tapping Mode operation in air (See Chapter 10) and Contact AFM in
fluid (See Chapter 9).
11.2.1 Acknowledgments
Digital Instruments Veeco wishes to express its appreciation to the following
individuals for their assistance in preparing the following sections: Monika
Fritz, Manfred Radmacher, Magdalena Benzanilla, Helen G. Hansma, Jan H.
Ho, Craig B. Prater.
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