VWR XT96 - Page 27

27 vwr.com I Instruction manual VWR
®
PCR Cycler XT
96
'Elongation Time' and 'Melting Temp.'
The elongation time and the melting
temperature of the primers can be
calculated by using this button.
Calculated values are for guidance
only, and further optimization may be
necessary. Typically, the optimal
annealing temperature is about 5 °C
lower than the calculated melting
temperature.
Gradient step
Use this button to enter a temperature gradient.
The following dialogue appears:
Temp
Set the desired temperature for the
centre of the gradient.
Gradient ±
Set the desired temperature span of the
gradient. The resulting temperatures in
the 8 rows are shown on the buttons at
the left side of the window.
Time
Set the desired duration of the gradient step in hours, minutes and seconds.
Ramp Rate
Activate the checkbox if a custom ramp rate is desired for the step. The setting here will
overwrite the 'Global Program Ramp' in the program options.
'Elongation Time' and 'Melting Temp.'
See above - Temperature.
Cycle
Use this button to define a program
cycle (loop), consisting of several
individual program steps.
In the field 'Number of Cycles' enter the
desired number of cycle repeats
, (max.
99 cycles).