Contents ▲ 109 ▼ Index
Protein Absolute Quantitation
Absolute quantitation is calculated based on the relative concentration of a sample and
the user-defined standard and the known concentration of this user-defined standard.
For protein samples you can enable the use of calibration for each sample and enter the
concentration of the standard protein. This allows you to generate a calibration curve,
which can be used to analyze and quantitate this protein within different samples on the
same chip.
Using Calibration in Protein Assays
The calibration feature for protein assays allows absolute quantitation based on external
standard calibration.
On the Chip Summary tab, use the sample table on the Sample Information sub-tab to
define the samples that you want to use as calibration standards and enter a
concentration.