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Agilent Technologies 2100 - Analyzing and Evaluating the Results of an Electrophoretic Assay

Agilent Technologies 2100
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Contents 81 Index
Analyzing and Evaluating the Results of an Electrophoretic Assay
The purpose of electrophoretic assays is to separate sample components and determine
their size, concentration, purity, or molarity. Results for a particular sample are calculated
after all data for that sample has been read.
The steps in data analysis differ depending on the type of assay in use:
Data Analysis: DNA” on page 82
Data Analysis: RNA and Cy5-Labeled Nucleic Acids” on page 87
The RNA Integrity Number (RIN)” on page 90
Data Analysis: Protein” on page 104
Smear Analysis” on page 112
Further steps in analysis are:
Changing the Data Analysis” on page 116
Manual Integration” on page 131
Reanalyzing a Chip Data File” on page 141
Comparing Samples from Different Electrophoretic Chip Runs” on page 143
How to Use the Form Mode” on page 159

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