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Agilent Technologies 2100 - Analyzing and Evaluating the Results of an Electrophoretic Assay

Agilent Technologies 2100
366 pages
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Contents ▲ 81 ▼ Index
Analyzing and Evaluating the Results of an Electrophoretic Assay
The purpose of electrophoretic assays is to separate sample components and determine
their size, concentration, purity, or molarity. Results for a particular sample are calculated
after all data for that sample has been read.
The steps in data analysis differ depending on the type of assay in use:
• “Data Analysis: DNA” on page 82
• “Data Analysis: RNA and Cy5-Labeled Nucleic Acids” on page 87
• “The RNA Integrity Number (RIN)” on page 90
• “Data Analysis: Protein” on page 104
• “Smear Analysis” on page 112
Further steps in analysis are:
• “Changing the Data Analysis” on page 116
• “Manual Integration” on page 131
• “Reanalyzing a Chip Data File” on page 141
• “Comparing Samples from Different Electrophoretic Chip Runs” on page 143
• “How to Use the Form Mode” on page 159