Contents ▲ 211 ▼ Index
Analyzing and Evaluating the Results of a Flow Cytometric Assay
You can analyze and evaluate result data of flow cytometric assays using either the dot
plot or the histogram view. In both views, you can evaluate the detected cells by defining
areas of interest.
• Histograms show the distribution of events related to the red and blue fluorescence
intensity. Gating is used to generate subsets based on markers in one histogram. See
“Using Histograms for Evaluation” on page 212 for detailed information.
• Dot plots show events as dots in a coordinate system where the blue fluorescence
value is related to the red. Regions and gates are used to determine the number of cells
with a fluorescence intensity lying in a defined range. See “Using Dot Plots for
Evaluation” on page 233 for detailed information.
If you use predefined assays, the markers and regions are set at the approximate position
where the events are expected. Refer to “Evaluating Antibody Staining, Apoptosis, and
GFP Assays” on page 242 for information on how to evaluate the predefined assays.
TIP
You can analyze and evaluate results while a chip run is still in progress.
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