Contents ▲ 179 ▼ Index
Preparing and Running a Flow Cytometric Assay
A flow cytometric chip run requires the following steps:
1. Switch on the Agilent 2100 bioanalyzer and start the 2100 expert software.
Details are given in “Starting 2100 Expert” on page 31.
2. Select a flow cytometric assay.
See “Selecting a Flow Cytometric Assay for a Chip Run” on page 181.
3. Prepare chip and samples.
Refer to “Preparing Samples and Chips for Flow Cytometric Assays” on page 185 and
to the appropriate Application Note and Reagent Kit Guide.
4. Load the chip into the bioanalyzer.
For details refer to “Loading the Cell Chip into the Bioanalyzer” on page 189.
5. Start the chip run.
This is described in “Running a Flow Cytometric Assay” on page 196.
When the chip run has finished, you can:
• Have a first look at the results (see “Displaying the Measurement Results (Flow
Cytometry)” on page 205).
• Document the chip run (see “Entering Chip, Sample, and Study Information” on
page 202).
To Next Page
Loading...
Need help?
General
