Contents ▲ 50 ▼ Index
Preparing and Running an Electrophoretic Assay
An electrophoretic chip run requires the following steps:
1. Switch on the Agilent 2100 bioanalyzer and start the 2100 expert software.
See “Starting 2100 Expert” on page 31.
2. Select an electrophoretic assay.
See “Selecting an Electrophoretic Assay for a Chip Run” on page 51.
3. Prepare reagents, chip, and samples.
See “Preparing Samples, Reagents, and Chips for Electrophoretic Assays” on page 54
and the appropriate Application Note or Reagent Kit Guide.
4. Load the chip into the bioanalyzer.
See “Loading the Electrophoresis Chip into the Bioanalyzer” on page 58.
5. Start the chip run.
See “Running an Electrophoretic Assay” on page 64.
When the chip run has finished, you can:
• Have a first look at the results (see “Displaying the Measurement Results
(Electrophoresis)” on page 72).
•
Document the chip run (see “Entering Chip, Sample, and Study Information” on page 69).
• Analyze and evaluate the results:
– See “Analyzing and Evaluating the Results of an Electrophoretic Assay” on page 81.
– See “Result Flagging” on page 152.
To Next Page
Loading...
Need help?
General
