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Preparing Samples, Reagents, and Chips for Electrophoretic Assays
Before you can load a chip, you have to prepare the samples and reagents. To find out
how to prepare the samples and reagents, refer to the various Reagent Kit Guides
available for each LabChip kit. Please refer to these documents for further information
and analytical specifications.
In general, preparing an electrophoretic assay involves the following steps:
• Check that you have everything that is listed in the appropriate Reagent Kit Guide.
Be aware that there can be small but important differences between the different
assays even for the same type of molecules (for example, between DNA 1000 and DNA
7500 assays).
• Make sure you are familiar with the essential measurement practices (see next page).
• Before running the first RNA assay: decontaminate the electrodes.
• Prepare all the reagent mixtures (for example, the gel-dye mix).
• Load the gel-dye mix using the priming station.
• Load the DNA/RNA marker solution and buffer.
• Load the destaining solution for protein assays.
• Load the chip with ladder and samples.
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