Contents ▲ 167 ▼ Index
Principles of Flow Cytometric Measurements
Besides electrophoretic assays (DNA, RNA, and proteins), the Agilent 2100 bioanalyzer
supports flow cytometric assays:
• First, cells are stained with two fluorescent dyes that correspond to biologically
relevant parameters, as described in the application notes available for each assay.
“Staining Cells” on page 168 explains the principle.
• Next, the stained cells are analyzed on the chip. They pass the detector in single file and
are analyzed individually for their red and blue fluorescence intensities. The results are
displayed as histograms or dot plots. Refer to “Cell Detection with the Agilent 2100
Bioanalyzer” on page 170 for a detailed explanation.
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