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Staining Cells
With the 2100 expert software, you can differentiate several properties of a cell. The
characteristics that are examined depend on the dye, which binds specifically to a
cellular constituent or is metabolized by the cell to generate a fluorescent product. You
usually use two dyes with different colors. Typically, one of the two dyes is used as
reference dye to select the target cells (living/dead, cell line type, etc.). The second dye
can be used to detect another characteristic of the cell.
Recommended dyes
The tables below list dyes that match to the detection optics specification (Excitation
max: 470 & 630 nm; Emission max: 525 & 680 nm).
The following dyes are recommended for use as the blue stain:
Dye (blue fluorescence) Max. Excitation
wavelength
Max. Emission
wavelength
Calcein (living cell stain) 493 nm 514 nm
Cell Tracker green (cell tracing,
viability stain)
492 nm 517 nm
GFP (green fluorescent protein) 490 nm 510 nm
SYTO16 (DNA dye) 485 nm 530 nm
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