Contents ▲ 87 ▼ Index
Data Analysis: RNA and Cy5-Labeled Nucleic Acids
The data analysis process for RNA and the Cy5-labeled nucleic acids assays consists of
the following steps:
1. Raw data is read and stored by the system for all of the individual samples.
2. The data is filtered and the resulting electropherograms of all samples are plotted. You
can change the settings of the data analysis after the run and reanalyze your data.
3. Fragments are identified for all samples and tabulated by peak ID. You can change the
settings of the peak find algorithm for any or all samples and reanalyze the data.
4. An RNA ladder (containing a mixture of RNA of known concentration) is run first (see
the electropherogram below). The concentrations and sizes of the individual base pairs
are preset in the assay and cannot be changed.
Electropherogram of RNA 6000 Ladder (Ambion, Inc. cat. no. 7152)
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