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8. To calculate the concentration of the individual DNA fragments in all sample wells, the
upper marker, in conjunction with an assay-specific concentration against base-pair
size calibration curve, is applied to the individual sample peaks in all sample wells.
NOTE
The software allows you to redefine the peaks chosen as upper and lower markers. A
change in marker selection will cause quantitative changes in the calibration
procedure, however, and therefore in the entire data evaluation.
9. If the check box Rest. Digest on the Chip Summary Tab is enabled, the 2100 expert
software flags peaks that may have co-migrated:
Since it is assumed that the molarity of all the fragments in a restriction digest should
be the same, any peaks or clusters having a molarity that is significantly larger than the
rest are flagged as potentially co-migrating peaks, allowing you to examine them in
more detail.
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