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Smear Analysis
The 2100 expert software allows to perform a smear analysis for all electrophoresis
assays.
When the smear analysis is enabled, the software allows you to define regions of
interest. These regions are used to define the area of broad peaks and determine their
part of the total area. Smear analysis provide a means to analyze broad signals that can
be hardly evaluated with the normal peak assignment.
You therefore can define regions of interest that contain the peaks (base pair size) that
you are interested in. For these regions you can determine the covered area in relation to
the total area.
Enabling Smear Analysis
To enable smear analysis:
1 Go to the Electropherogram tab in the Data context:
2 Go to the setpoint explorer and select the Local or Global tab, depending on which
samples should be analyzed.
3 Select the Advanced mode.